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研 究 概 要

稲作地域の拡大-開花期のデザイン育種

イネは、日の長さが短くなると開花する(イネでは開花を出穂といいます)短日植物です。イネの品種がもつ様々な出穂特性は、稲作地域の拡大と収量の安定化に大きく貢献しています。私たちは、ガンマ線照射によって誘発された出穂期に関する変異体を利用して、イネにおける出穂の分子機構とそれに関わる遺伝子のネットワークを研究しています。ここから得られる知見は、様々な地域に適応した出穂特性をもつ品種を効率よく育成する鍵となります。


論 文 発 表 (*: Corresponding author)

The effects of phytochrome-mediated light signals on the developmental acquisition of photoperiod sensitivity in rice
Yoshitake Y, Yokoo T, Saito H*, Tsukiyama T, Xu Q, Zikihara K, Katsura H, Tokutomi S, Abosi T, Mori N, Inoue H, Nishida H, Kohchi T, Teraishi M, Okumoto Y, Tanisaka T
Sci. Rep. (2015) 5: 7709


Se14, encoding a JmjC domain-containing protein, plays key roles in long-day suppression of rice flowering through the demethylation of H3K4me3 of RFT1.
Yokoo T, Saito H*, Yoshitake Y, Xu Q, Asami T, Tsukiyama T, Teraishi M, Okumoto Y & Tanisaka T
PLOS One (2014) 9(4): e96064.


The effects of the photoperiod-insensitive alleles, se13, hd1 and ghd7, on yield components in rice.
Xu Q, Saito H, Hirose I, Katsura K, Yoshitake Y, Yokoo T, Tsukiyama T, Teraishi M, Tanisaka T & Okumoto Y*
Mol. Breeding (2013) 33(4): 813-819.


Ef7 encodes an ELF3-like protein and promotes rice flowering by negatively regulating the floral repressor gene Ghd7 under both short- and long-day conditions.
Saito H, Ogiso-Tanaka E, Okumoto Y*, Yoshitake Y, Izumi H, Yokoo T, Matsubara K, Hori K, Yano M, Inoue H, &Tanisaka T
Plant Cell Phsyiol (2012) 53(4): 717-728.


Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene.
Saito H, Okumoto Y. Yoshitake Y, Inoue H, Yuan Q, Teraishi M, Tsukiyama T, Nishida H & Tanisaka T*
Theor. Appl. Genet. (2011) 122(1): 109-118.


Multiple alleles at Early flowering 1 locus making variation in the basic vegetative growth period in rice (Oryza sativa L.).
Saito H, Yuan Q, Okumoto Y, Doi K, Yoshimura A, Inoue H, Teraishi M, Tsukiyama T & Tanisaka T*
Theor. Appl. Genet. (2009) 119: 315-323.


Identification of a novel gene ef7 conferring an extremely long basic vegetative growth phase in rice.
Yuan Q, Saito H, Okumoto Y, Inoue H, Nishida H, Tsukiyama T, Teraishi M & Tanisaka T*
Theor. Appl. Genet. (2009) 119: 675-684.


Heading Time Genes Responsible for the Regional Adaptability of 'Tongil-type Short-culmed Rice Cultivars' Developed in Korea.
Saito H, Okumoto Y, Teraishi M, Yuan Q, Nakazaki T & Tanisaka T*
Breed. Sci. (2007) 57(2): 135-143.

A novel gene ef1-h conferring an extremely long basic vegetative growth period in rice.
Nishida H, Inoue H, Okumoto Y and Tanisaka T*
Crop Sci. (2002) 42(2): 348-354.


Interactive effects of two heading-time loci, Se1 and Ef1, on pre-flowering developmental phases in rice (Oryza sativa L.).
Ichitani K, Inoue H, Nishida H, Okumoto Y & Tanisaka T*
Euphytica (2002) 123(2): 227-234.

Analysis of tester lines for rice (Oryza sativa L.) heading-time genes using reciprocal photoperiodic transfer treatments.
Nishida H, Okumoto Y, Nakagawa H, Ichitani K, Inoue H & Tanisaka T*
Ann. Bot. (2001) 88(4): 527-536.


トランスポゾンによる生物進化の解明と育種への応用

すべての生物のゲノム中には、MITEと呼ばれる小さなトランスポゾンが多数存在します。MITEは、ゲノム中を飛び回ることで進化に不可欠な自然突然変異を誘発してきました。私たちは、mPingと名付けたイネのMITEに着目し、MITEがどのようにしてゲノム進化や種分化、生態型分化に貢献してきたのかを研究しています。また、有用形質をもつ品種の効率的な選抜や育成にmPingを利用するための研究を行っています。


論 文 発 表 (*: Corresponding author)

The active miniature inverted-repeat transposable element mPing post-transcriptionally produces new transcriptional variants in the rice genome.
Kum R, Tsukiyama T*, Inagaki H, Saito H, Teraishi M, Okumoto Y & Tanisaka T
Mol. Breed. (2015) (in print)


Early embryogenesis-specific expression of the rice transposon Ping Enhances amplification of the MITE mPing.
Teramoto S, Tsukiyama T*, Okumoto Y & Tanisaka T
PLOS Genet. (2014) 10(6): e1004396.


Loss-of-function of a ubiquitin-related modifier promotes the mobilization of the active MITE mPing.
Tsukiyama T, Teramoto S, Yasuda K, Horibata A, Mori N, Okumoto Y, Teraishi M, Saito H, Onishi A, Tamura K & Tanisaka T*
Mol. Plant (2013) 6(3): 790-801.


Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice.
Yasuda K, Ito M, Sugita T, Tsukiyama T, Saito H, Naito K, Teraishi M, Tanisaka T & Okumoto Y*
Mol Breeding (2013) 32(3): 505-516.


Mobilization of the active transposon mPing in interspecific hybrid rice between Oryza sativa and O. glaberrima.
Yasuda K, Tsukiyama T*, Karki S, Okumoto Y, Teraishi M, Saito H & Tanisaka T
Euphytica (2013) 192(1): 17-24.


Unexpected consequences of a sudden and massive transposon amplification on rice gene expression.
Naito K, Zhang F, Tsukiyama T, Saito H, Hancock CN, Richardso AO, Okumoto Y, Tanisaka T* & Wessler SR*
Nature (2009) 461: 1130-1134


Analysis of distribution and proliferation of mPing family transposons in a wild rice (Oryza rufipogon Griff.).
Karki S, Tsukiyama T, Okumoto Y, Rizal G, Naito K, Teraishi M, Nakazaki T & Tanisaka T*
Breed. Sci. (2009) 59: 267-307.


High potential of a transposon mPing as a marker system in japonica x japonica cross in rice.
Monden Y, Naito K, Okumoto Y, Saito H, Oki N, Tsukiyama T, Ideta O, Nakazaki T, Wessler SR & Tanisaka T*
DNA Res. (2009) 16: 131-140.


A genome-wide view of miniature inverted-repeat transposable elements (MITEs) in rice, Oryza sativa ssp. Japonica.
Oki N, Yano K, Okumoto Y, Tsukiyama T, Teraishi M & Tanisaka T*
Genes Genet. Syst. (2008) 83: 321-329.


Dramatic amplification of a rice transposable element during recent domestication.
Naito K, Cho E, Yang G, Campbell MA, Yano K, Okumoto Y, Tanisaka T & Wessler SR*
P. Natl. Acad. Sci. USA (2006) 103(47): 17620-17625.


Mobilization of a transposon in the rice genome.
Nakazaki T, Okumoto Y, Horibata A, Yamahira S, Teraishi M, Nishida H, Inoue H, Tanisaka T*
Nature (2003) 421: 170-172.


ダイズを食べて健康に

ダイズには生活習慣病予防効果の高いタンパク質、骨粗鬆症や癌抑制に効果があるイソフラボンなどの機能性成分が多く含まれています。最新の遺伝情報を利用して日本の伝統的なダイズをさらにおいしく、健康食品としての価値をさらに高くするための研究を行っています。また、雨の多い日本において安定生産ができるように冠水抵抗性を付与するための研究を行っています。


論 文 発 表 (*: Corresponding author)

Wide genetic variation in phenolic compound content of seed coats among black soybean cultivars.
Phommalath S, Teraishi M, Yoshikawa T, Saito H, Tsukiyama T, Nakazaki T, Tanisaka T & Okumoto Y*
Breed. Sci. (2014) 64: 409-415.


Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties.
Yoshikawa T, Okumoto Y*, Ogata D, Sayama T, Teraishi M, Terai M, Toda T, Yamada K, Yagasaki K, Yamada N, Tsukiyama T, Yamada T & Tanisaka T
Breed. Sci. (2010) 60(3): 243-254.


QTL analysis of seed-flooding tolerance in soybean (Glycine max [L.] Merr.).
Sayama T, Nakazaki T, Ishikawa G, Tagasaki K, Yamada N, Hirota N, Hirata K, Yoshikawa T, Saito H, Teraishi M, Okumoto Y, Tsukiyama T & Tanisaka T*
Plant Sci. (2009) 176: 514-521.


その他の研究

論 文 発 表 (*: Corresponding author)

植物のメタボロームから育種を考える

The tyrosine aminomutase TAM1 is required for beta-tyrosine biosynthesis in rice.
Yan j, Aboshi T, Teraishi M, Strickler SR, Spindel JE, Tung CW, Takata R, Matsumoto F, Maesaka Y, McCouch SR, Okumoto Y, Mori N, Jander G*
Plant Cell (2015) 27(4): 1265-1278.



コムギの根系を制御する遺伝機構を明らかにする

Novel QTLs for growth angle of seminal roots in wheat (Triticum aestivum L.)
Hamada A, Nitta M, Nasuda S, Kato K, Fujita M, Matsunaka H, Okumoto Y*
Plant Soil (2012) 354(1-2): 395-405.



真核生物に広く保存されたユビキチン様タンパク質の機能に迫る

Transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts.
Hamamoto Y, Tsukiyama T*, Yoshitake Y, Teraishi M, Okumoto Y & Tanisaka T
Biosci. Biotechnol. Biochem. (2013) 77(12): 2480-2482.


Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1.
Tsukiyama T, Lee J, Okumoto Y, Teraishi M, Tanisaka T & Inouye K*
Biosci. Biotechnol. Biochem. (2010) 74(2): 430-432.



植物におけるキチン分解酵素の役割を解明する

Gene cloning, expression, purification and characterization of rice (Oryza sativa L.) class II chitinase CHT11.
Xayphakatsa K, Tsukiyama T, Inouye K*, Okumoto Y, Nakazaki T & Tanisaka T
Enzyme Microb. Tech. (2008) 43: 19-24.


Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.).
Nakazaki T, Tsukiyama T, Okumoto Y, Kageyama D, Naito K, Inouye K & Tanisaka T*
Genome (2006) 49: 619-630.