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CROPS ARE OUR BEST FRIENDS
-toward developing new varieties-

Plant breeding is to produce new crop varieties with improved genetic nature, which has contributed to the development of human beings. Plant Breeding Science is a field that provides useful knowledge on genetic improvement of crops. Our major subjects are to identify and isolate useful genes for agronomic traits in rice, wheat and soybean, and to elucidate the transposition mechanism and evolutional role of the transposon mPing family. For this purpose, we are enthusiastically conducting experiments not only in laboratory but also in field.
'Fossil' gene is actively transposing in rice!!

Small DNA fragments, called MITEs, make up a large portion of the genomes of all organisms. Because of their molecular structures, MITEs have been thought to be a family of transposons, which induce spontaneous mutations and contribute to evolution. Analyzing curious chimaeric panicles of rice (picture 1), we revealed that MITEs are not fossil genes but are active transposons moving in genomes even now. This is the first finding of active MITEs in animals and plants, and provides an important clue for elucidation of organic evolution and functional analysis of rice genes.

Chimaeric panicle caused by the transposition of MITE
Soybean can make you healthy!!

Genetic map of SSR markers in soybean

Soybean has many functional substances, such as isoflavone, preventing diseases caused by life styles and cancer. Utilizing the latest genetic information, we attempt to make soybean, a Japanese traditional crop, more delicious and worthy as a functional food. In addition, we investigate the genetic mechanism of seed flooding tolerance necessary for the stable production in rainy climate regions, such as Japan.

Baking delicious bread using Japanese wheat!!

Bread wheat is not adapted to the wet climatic conditions in Japan, because it originated in the arid climate in the Mideast. In addition, bread wheat was used for making noodles in Japan until recently. For these reasons, few Japanese wheat varieties are suitable for baking bread. We are aiming at identifying the genetic factors that contribute to the improvement of baking quality of Japanese wheat.


Differences in baking quality among wheat varieties
Keywords

rice, soybean, wheat, plant breeding, genetic analysis, agronomic character, mutation, transposon, heading date, flooding tolerance, pathogenesis-related protein, transgenic plant

Recent publications

The effects of phytochrome-mediated light signals on the developmental acquisition of photoperiod sensitivity in rice.
Yoshitake Y, Yokoo T, Saito H*, Tsukiyama T, Xu Q, Zikihara K, Katsura H, Tokutomi S, Abosi T, Mori N, Inoue H, Nishida H, Kohchi T, Teraishi M, Okumoto Y, Tanisaka T
Sci. Rep. (2015) 5: 7709.

Wide genetic variation in phenolic compound content of seed coats among black soybean cultivars.
Phommalath S, Teraishi M, Yoshikawa T, Saito H, Tsukiyama T, Nakazaki T, Tanisaka T & Okumoto Y*
Breed. Sci. (2014) 64: 409-415.

Early embryogenesis-specific expression of the rice transposon Ping Enhances amplification of the MITE mPing.
Teramoto S, Tsukiyama T*, Okumoto Y & Tanisaka T
PLOS Genet. (2014) 10(6): e1004396.

Se14, encoding a JmjC domain-containing protein, plays key roles in long-day suppression of rice flowering through the demethylation of H3K4me3 of RFT1.
Yokoo T, Saito H*, Yoshitake Y, Xu Q, Asami T, Tsukiyama T, Teraishi M, Okumoto Y & Tanisaka T
PLOS One (2014) 9(4): e96064.

Induced mutations will help mold the future of food.
Tsukiyama T, Saito H, Naito K, Teraishi M, Monden Y, and Tanisaka T
Gamma Field Symp. (2013)

Transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts.
Hamamoto Y, Tsukiyama T*, Yoshitake Y, Teraishi M, Okumoto Y & Tanisaka T
Biosci. Biotechnol. Biochem. (2013) 77(12): 2480-2482.

Loss-of-function of a ubiquitin-related modifier promotes the mobilization of the active MITE mPing.
Tsukiyama T, Teramoto S, Yasuda K, Horibata A, Mori N, Okumoto Y, Teraishi M, Saito H, Onishi A, Tamura K & Tanisaka T*
Mol. Plant (2013)6(3): 790-801.

The effects of the photoperiod-insensitive alleles, se13, hd1 and ghd7, on yield components in rice.
Xu Q, Saito H, Hirose I, Katsura K, Yoshitake Y, Yokoo T, Tsukiyama T, Teraishi M, Tanisaka T & Okumoto Y*
Mol. Breeding (2013) 33(4): 813-819.

Utilization of transposable element mPing as a novel genetic tool for modification of the stress response in rice.
Yasuda K, Ito M, Sugita T, Tsukiyama T, Saito H, Naito K, Teraishi M, Tanisaka T & Okumoto Y*
Mol Breeding (2013) 32(3): 505-516.

Mobilization of the active transposon mPing in interspecific hybrid rice between Oryza sativa and O. glaberrima.
Yasuda K, Tsukiyama T*, Karki S, Okumoto Y, Teraishi M, Saito H & Tanisaka T
Euphytica (2013) 192(1): 17-24.

Ef7 encodes an ELF3-like protein and promotes rice flowering by negatively regulating the floral repressor gene Ghd7 under both short- and long-day conditions.
Saito H, Ogiso-Tanaka E, Okumoto Y*, Yoshitake Y, Izumi H, Yokoo T, Matsubara K, Hori K, Yano M, Inoue H & Tanisaka T
Plant Cell Phsyiol (2012) 53(4): 717-728.

Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene.
Saito H, Okumoto Y. Yoshitake Y, Inoue H, Yuan Q, Teraishi M, Tsukiyama T, Nishida H & Tanisaka T*
Theor. Appl. Genet. (2011) 122(1): 109-118.

Gene cloning, bacterial expression, and purification of a novel rice (Oryza sativa L.) ubiquitin-related protein, RURM1.
Tsukiyama T, Lee J, Okumoto Y, Teraishi M, Tanisaka T & Inouye K*
Biosci. Biotechnol. Biochem. (2010) 74(2): 430-432

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties.
Yoshikawa T, Okumoto Y*, Ogata D, Sayama T, Teraishi M, Terai M, Toda T, Yamada K, Yagasaki K, Yamada N, Tsukiyama T, Yamada T & Tanisaka T
Breed. Sci. (2010) 60(3): 243-254.

Unexpected consequences of a sudden and massive transposon amplification on rice gene expression.
Naito K, Zhang F, Tsukiyama T, Saito H, Hancock CN, Richardso AO, Okumoto Y, Tanisaka T* & Wessler SR*
Nature (2009) 461: 1130-1134

Analysis of distribution and proliferation of mPing family transposons in a wild rice (Oryza rufipogon Griff.).
Karki S, Tsukiyama T, Okumoto Y, Rizal G, Naito K, Teraishi M, Nakazaki T & Tanisaka T*
Breed. Sci. (2009) 59: 267-307.

High potential of a transposon mPing as a marker system in japonica x japonica cross in rice.
Monden Y, Naito K, Okumoto Y, Saito H, Oki N, Tsukiyama T, Ideta O, Nakazaki T, Wessler SR & Tanisaka T* DNA Res. (2009) 16: 131-140.

Multiple alleles at Early flowering 1 locus making variation in the basic vegetative growth period in rice (Oryza sativa L.).
Saito H, Yuan Q, Okumoto Y, Doi K, Yoshimura A, Inoue H, Teraishi M, Tsukiyama T & Tanisaka T*
Theor. Appl. Genet. (2009) 119: 315-323.

Identification of a novel gene ef7 conferring an extremely long basic vegetative growth phase in rice.
Yuan Q, Saito H, Okumoto Y, Inoue H, Nishida H, Tsukiyama T, Teraishi M & Tanisaka T*
Theor. Appl. Genet. (2009) 119: 675-684.

Identification and characterization of the erect-pose panicle gene EP conferring high grain yield in rice (Oryza sativa L.).
Wang J, Nakazaki T, Chen S, Chen W, Saito H, Tsukiyama T, Okumoto Y, Xu Z* & Tanisaka T*
Theor. Appl. Genet. (2009) 119:85-91.

QTL analysis of seed-flooding tolerance in soybean (Glycine max [L.] Merr.).
Sayama T, Nakazaki T, Ishikawa G, Tagasaki K, Yamada N, Hirota N, Hirata K, Yoshikawa T, Saito H, Teraishi M, Okumoto Y, Tsukiyama T & Tanisaka T*
Plant Sci. (2009) 176: 514-521.

A genome-wide view of miniature inverted-repeat transposable elements (MITEs) in rice, Oryza sativa ssp. Japonica.
Oki N, Yano K, Okumoto Y, Tsukiyama T, Teraishi M & Tanisaka T*
Genes Genet. Syst. (2008) 83: 321-329.

Gene cloning, expression, purification and characterization of rice (Oryza sativa L.) class II chitinase CHT11.
Xayphakatsa K†, Tsukiyama T†, Inouye K, Okumoto Y, Nakazaki T & Tanisaka T*
Enzyme Microb. Tech. 4(2008) 3: 19-24.

Dramatic amplification of a rice transposable element during recent domestication.
Naito K, Cho E, Yang G, Campbell MA, Yano K, Okumoto Y, Tanisaka T & Wessler SR*
P. Natl. Acad. Sci. USA (2006) 103(47): 17620-17625.

Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.).
Nakazaki T, Tsukiyama T, Okumoto Y, Kageyama D, Naito K, Inouye K & Tanisaka T*
Genome (2006) 49(6):619-630